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Key Documents

54327

Sigma-Aldrich

Lipase, immobilized on Immobead 150 from Pseudomonas cepacia

≥900 U/g

Sinónimos:

Immobilized Lipase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Pseudomonas cepacia)

Quality Level

form

powder (or beads)

specific activity

≥900 U/g

color

white to off-white

application(s)

life science and biopharma

storage temp.

2-8°C

Gene Information

Burkholderia spp. ... BURCE16_RS29975(56665765)

General description

Research area: Cell Signaling

Lipases are water-soluble, ester hydrolases. Long aliphatic chain acyl esters of cholesterol (cholesteryl esters), triacyl esters of glycerol (triacylglycerols), acyl esters of long chain alcohols (wax esters), diacyl esters of glycerol (diacylglycerols), and monoacyl esters of glycerol, are some of the common substrates for lipases.

Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.Lipase, immobilized on Immobead 150 from Pseudomonas cepacia has been used in enzymatic hydrolysis of polylactic acid (PLA). It has also been used as biocatalysts in lipase-catalyzed kinetic resolution reactions.

Biochem/physiol Actions

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.Lipases are known to regulate energy metabolism of the cell. Lipolysis is highly essential for cellular uptake or release of fatty acids (FAs) and glycerol. Gastrointestinal lipolysis catabolizes dietary fat. Vascular lipolysis hydrolyzes lipoprotein-related triglycerides (LTPs) in the blood. In the intracellular domain, lipolysis catalyzes the breakdown of triglycerides (TGs) stored in LDLs for exportation of fatty acids (fat) (fat tissue) or metabolism (non-adipose tissue).

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Studies on the chemoenzymatic synthesis of (R)-and (S)-methyl 3-aryl-3-hydroxypropionates: the influence of toluene-pretreatment of lipase preparations on enantioselective transesterifications
Borowiecki P & Bretner M
Tetrahedron Asymmetry (2013)
Florian Golombek et al.
ACS omega, 6(43), 29192-29200 (2021-11-09)
Deracemizations are clearly preferable to kinetic resolutions in the production of chiral molecules from racemates, as they allow up to 100% chemical and optical yield. Here we present a new process route for multienzymatic deracemizations that is relevant for reaction
Fatty Acid Signaling: The New Function of Intracellular Lipases
Papackova Z & Cahova N
International Journal of Molecular Sciences, 3831?3855-3831?3855 (2015)
Rudolf Zechner et al.
Cell metabolism, 15(3), 279-291 (2012-03-13)
Lipolysis is defined as the catabolism of triacylglycerols stored in cellular lipid droplets. Recent discoveries of essential lipolytic enzymes and characterization of numerous regulatory proteins and mechanisms have fundamentally changed our perception of lipolysis and its impact on cellular metabolism.
Lipases
H.L. Brockman
Encyclopedia of Biological Chemistry, 729-732 (2013)

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