10663573001
Roche
BM-Condimed
suitable for cell culture, solution, pkg of 100 mL
Sinónimos:
growth factor
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About This Item
UNSPSC Code:
12352207
Productos recomendados
sterility
0.2 μm filtered
form
solution
packaging
pkg of 100 mL
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
solubility
water: miscible
General description
BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
Application
BM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes.
Physical form
Physiological solution, filtered through a 0.2 μm pore size membrane, buffered with sodium bicarbonate.
Preparation Note
Working concentration: 1 to 100μg/ml
1 to 100μg/ml
Working solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells.
Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.
1 to 100μg/ml
Working solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture.
Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells.
Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines.
Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells.
Storage and Stability
Store at 2 to 8 °C. (Store in dark!)
Other Notes
For life science research only. Not for use in diagnostic procedures.
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