5-Lipoxygenase, Human, Recombinant, S. fruigiperda
5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4.
Sinónimos:
5-LO
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Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Advertencia
Toxicity: Standard Handling (A)
Definición de unidad
One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
Forma física
In 100 mM Tris containing 5 mM EGTA, pH 8.0.
Reconstitución
Following initial thaw, aliquot and freeze (-70°C).
Otras notas
Brock, T.G., et al. 1997. J. Biol. Chem.272, 8276. Pouliot, M., et al. 1996. Eur. J. Biochem.238, 250. Zhang, Y.Y., et al. 1993. J. Biol. Chem.268, 2535. Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA81, 689.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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