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Merck

D199303

Sigma-Aldrich

2,4-Dinitrofenilhidracina

reagent grade, 97%

Sinónimos:

(2,4-dinitrophenyl)-hydrazine, DNPH

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About This Item

Fórmula lineal:
(O2N)2C6H3NHNH2
Número de CAS:
Peso molecular:
198.14
Beilstein/REAXYS Number:
615586
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.21
grade:
reagent grade
assay:
97%
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grade

reagent grade

Quality Level

assay

97%

contains

33% water (minimum)

mp

197-200 °C (lit.)

functional group

hydrazine
nitro

SMILES string

NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2

InChI key

HORQAOAYAYGIBM-UHFFFAOYSA-N

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General description

La 2,4-dinitrofenilhidracina (DNPH), un derivado de la hidracina, es un posible mutágeno. Se ha analizado su estructura molecular y cristalina.[1] Los enlaces de hidrógeno intramoleculares e intermoleculares en la DNPH han sido investigados mediante análisis espectroscópico vibracional.[2] El mecanismo de degradación isotérmica de la DPHN ha sido estudiado por calorimetría diferencial de barrido (DSC).[3]

Application

La 2,4-dinitrofenilhidracina se ha utilizado como reactivo para detectar la presencia de aldehídos y cetonas en los carbonilos proteicos.[4][5][6] Puede utilizar como reactivo colorimétrico en la estimación cuantitativa de la prednisona.[7]

Packaging

500 g contiene un peso total del producto húmedo de ~485 g. La diferencia se debe a la adición de agua para cumplir con los requisitos del DOT.

Pictograms

FlameExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 4 Oral - Desen. Expl. 1

supp_hazards

Storage Class

4.1A - Other explosive hazardous materials

wgk_germany

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Certificados de análisis (COA)

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Visite la Librería de documentos

2,4-dinitrophenylhydrazine: a sensitive quantitative reagent for determining microgram quantities of prednisone.
A L Woodson
Journal of pharmaceutical sciences, 60(10), 1538-1542 (1971-10-01)
Kinetic analysis of isothermal decomposition of 2, 4-dinitrophenylhydrazine using differential scanning calorimetry.
Musuc AM, et al.
Thermochimica Acta, 448(2), 130-135 (2006)
Chem. Abstr., 118, 22127d-22127d (1993)
Vibrational spectroscopy and theoretical studies on 2, 4-dinitrophenylhydrazine.
Chis V, et al.
Journal of Molecular Structure, 744, 363-368 (2005)
J. Chem. Soc. Pak., 14, 73-73 (1992)

Questions

1–4 of 4 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Before use, is it necessary to dry off the water content in this reagent? If so, what would be the temperature and how long without triggering degradation of the reagent?

    1 answer
    1. This product is intentionally provided as a moist powder, with at least 33% water by weight. The material may be safely handled in the moistened state, but not as a dry powder. The recommendation would be to NOT dry off the water content.

      Helpful?

  4. We have this product (D199303-500G) on our site and want to dispose of it . Can you please give us instructions on how to dispose the product safely ?

    1 answer
    1. Please refer to section 13 of the Safety Data Sheet for disposal information. Consult with facility or municipal EHS authorities to assure proper guidelines are followed. See the link below to review the SDS:
      https://www.sigmaaldrich.com/sds/aldrich/d199303

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