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Merck

200182

Sigma-Aldrich

Nitrazine Yellow

indicator grade, Dye content 85 %

Sinónimos:

2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt, 2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt, Nitrazol Yellow

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About This Item

Fórmula empírica (notación de Hill):
C16H8N4Na2O11S2
Número de CAS:
Peso molecular:
542.36
Colour Index Number:
14890
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

indicator grade

assay

>90.0% (HPLC)

form

powder

composition

Dye content, 85%

solubility

H2O: 1 mg/mL

λmax

586 nm

ε (extinction coefficient)

≥35000 at 587-591 nm in 0.1 M NaOH at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c(cc3cc(ccc13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H10N4O11S2.2Na/c21-16-11-3-2-10(32(26,27)28)5-8(11)6-14(33(29,30)31)15(16)18-17-12-4-1-9(19(22)23)7-13(12)20(24)25;;/h1-7,21H,(H,26,27,28)(H,29,30,31);;/q;2*+1/p-2/b18-17+;;

InChI key

XBLIFEQTVVSTIM-QIKYXUGXSA-L

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Application

Nitrazine Yellow has been used in dye-protein binding assay.[1]

Biochem/physiol Actions

Nitrazine Yellow ( 2-(2,4-dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt) is a pH-sensitive azo dye.[2] It changes color from yellow to blue in pH range 6.0–7.0 and is widely used in microscopy.[2][3] Throat swab immersed in nitrazine yellow is used to identify pH variations in the vagina before and after amniotomy.[4]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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[Group hypersensitivity between nitrazine yellow and pellidol (author's transl)].
M Novák
Ceskoslovenska dermatologie, 56(1), 22-24 (1981-02-01)
Shephali Tagore et al.
Journal of perinatal medicine, 38(6), 609-612 (2010-08-17)
to compare insulin-like growth factor binding protein-1 (IGFBP-1) (non-phosphorylated), placental alpha-microglobulin-1 (PAMG-1) and nitrazine test to diagnose premature rupture of membrane to allow gestation-specific management. we recruited 100 women with signs or symptoms of premature rupture of membranes (PROM), between
H Yamada et al.
The journal of obstetrics and gynaecology research, 24(2), 103-108 (1998-06-19)
A 24-year-old woman underwent conization and cervical cerclage during pregnancy. After she later complained of fluid leakage, a premature rupture of the membranes (PROM) was suspected, because of positive results on a test for nitrazine, the intra-amniotic dye injection method
P Ronnander et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 125, 54-63 (2018-09-18)
A mathematical model was developed to predict the transport of sumatriptan molecules across the skin followed by absorption into the bloodstream. The drug was encapsulated in dissolving polyvinylpyrrolidone-based microneedles shaped in the form of pyramids. Mass balance equations were derived
Ibrahim A Abdelazim
Archives of gynecology and obstetrics, 287(2), 205-210 (2012-09-13)
This study was designed to detect the accuracy of the fetal fibronectin (Quick Check fFN test(®)) in diagnosing premature rupture of fetal membranes. This comparative prospective study was carried out over 1 year in Ahmadi Kuwait Oil Company (KOC) Hospital

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