This product is not suitable for preserving or fixing phytoplankton. No strong iodine solutions are offered for sale due to regulatory issues. Only weak solutions of iodine are available, generally containing about 1% combined iodine and potassium iodide. Strong solutions suitable for parasitology or for use in preserving phytoplankton are not available.
32922
Lugol solution
according to Lugol
Synonym(s):
Iodine/Potassium iodide solution
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250 ML
MXP 1,568.00
1 L
MXP 5,089.00
About This Item
Recommended Products
form
liquid
Quality Level
quality
according to Lugol
density
1.006 g/cm3 at 20 °C
application(s)
diagnostic assay manufacturing
food and beverages
hematology
histology
storage temp.
room temp
SMILES string
[K+].[I-].II
InChI
1S/I2.HI.K/c1-2;;/h;1H;/q;;+1/p-1
InChI key
XUEKKCJEQVGHQZ-UHFFFAOYSA-M
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General description
Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology.[1] It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.
Application
- Lugol′s solution is generally used as a mordant in Gram staining, and for fixing invertebrate blood cells and protozoa.[1]
- It is suitable for the detection of starch in plants.
- It has been used in a study to assess narrow-band imaging without image magnification for detecting high-grade dysplasia and intramucosal esophageal squamous cell carcinoma.
- It has been used in a study to determine its benefit in aiding the detection, presence, and spread of small squamous cell carcinomas of the esophagus.
Biochem/physiol Actions
Iodine diffuses rapidly through hydrophilic and hydrophobic domains of tissues and loosely binds to proteins, staining them brown. It reacts irreversibly with unsaturated lipid linkages and with tyrosine and histidine aromatic rings.[1]
Features and Benefits
- Ready-to-use solution.
Principle
In bacteriology, Gram staining allows rapid differentiation between Gram-positive and Gram-negative bacteria. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with the primary stain, crystal violet. Lugol solution is added thereafter as the mordant, forming crystal violet-iodine complexes. During the decolorization, these complexes stay entrapped in the multilayer mureine structure of the cell wall of Gram-positive bacteria which appear blue-violet. Gram-negative bacteria have a single-layered cell wall that does not retain the dye-iodine complexes and are counterstained with safranine solution, appearing pink.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Find documentation for the products that you have recently purchased in the Document Library.
Histological and Histochemical Methods: Theory and Practice
J.A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Jason M Cole et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6335-6340 (2018-06-07)
In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources
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Which Lugol`s solution for phytoplankton preservation sample used?
1 answer-
Helpful?
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Buenas tardes. Por favor, pueden especificar las concentraciones de I2 y KI en las siguientes soluciones de Lugol (32922, 62650, y L6146)? No encuentro la información en su página web. Gracias
1 answer-
The composition of product 32922 is considered proprietary. The exact percentages of I2 and KI cannot be stated. The SDS for 32922 does state the composition for the Iodine is between 0.1% to less than 1%. This would suggest the composition of 32922 is comparable other standard "weak Lugol" formulations. Products L6146 and 62650 can be classified as "weak Lugol" formulations. Product L6146 and 62650 are formulated to contain approximately 0.35% Iodine and 0.70% Potassium Iodide. While the concentration of L6146 is a bit higher at 0.37% Iodine and 0.74% Potassium Iodide, there is no functional difference between these two products.
There are "strong" Lugol formulations that can approach a combined I2 and KI concentration of between 10 to 15%. These "strong" formulations are intended for use in procedures for parasitology. 32922, 62650 and L6146 are not suitable for use in procedures for parasitology.
Helpful?
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What are the differences between Lugol solution, products 32922 & 62650?
1 answer-
The composition of product 32922 is provided in the Certificate of Analysis, while for product 62650, only the density is reported. The formulations of the two products appear to be the same, but there is a difference in the amount of quality control testing performed and reported. Both products are intended for Research Use Only and should be viewed as "weak" Lugol Iodine solutions.
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