Product 03978 is not a GMP quality product. No testing is performed during Quality Control testing that measures lot to lot variability. The testing includes tests for Appearance, Thin Layer Chromatography, Density, Wavelength 1 at 656-666nm, Extinction Coefficient for Wavelength 1 and the solvent used to measure the wavelength and extinction coefficient. The information about a 1.5% concentration of dye is from the production documents used to manufacture the dye solution. The actual dye concentration is not measured.
03978
Methylene Blue solution
for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution
Synonym(s):
Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
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250 ML
MXP 1,464.00
About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
Beilstein:
3641570
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
grade
for microscopy
Quality Level
form
liquid
quality
concentrated, aqueous solution
shelf life
~3 yr
concentration
1.5% (1.5 g/ 100 mL)
density
1.0 g/mL at 20 °C
εmax
9.4 at 656-666 nm in water
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI
1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
InChI key
CXKWCBBOMKCUKX-UHFFFAOYSA-M
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General description
Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Application
Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability.[4] It has also been used to stain sections to determine the presence of mycobacteria.[5][6]
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Concerning the stated concentration of the product 03978 (Methylene blue solution, 1.5%), what is the uncertainty and lot-to-lot variability?
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