This antibody has not validated this antibody for reactivity to dsRNA from an IVT reaction. However, the following publication cites the use of this item to detect and ensure the removal of dsRNA contaminants from synthesized mRNA samples. Nat Commun. 2023 May 11;14(1):2657. https://pubmed.ncbi.nlm.nih.gov/37169757/
MABE1134
Anti-dsRNA Antibody, clone rJ2
culture supernatant, clone rJ2, from mouse
Synonym(s):
Double-stranded RNA
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.
Recommended Products
biological source
mouse
antibody form
culture supernatant
antibody product type
primary antibodies
clone
rJ2, monoclonal
species reactivity
virus
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
isotype
IgG2aκ
target post-translational modification
unmodified
General description
Double-stranded RNA (dsRNA) is a viral product that induces innate immunity, leading to the production of interferon (IFN) alpha and beta, which can lead to the activation of hundreds of IFN-stimulated genes that confer resistance to viruses. dsRNA of more than 30-bp length are reported to be is a key activator of the innate immune response against viral infections. dsRNA is produced by positive-strand RNA viruses, dsRNA viruses, and DNA viruses. However, negative-strand RNA viruses are not shown to generate any significant dsRNA signals. The interaction of the host cell with dsRNA can occur in several ways. Mainly, specific receptors activate the synthesis of IFN alpha and beta, antiviral proteins, and dsRNA-activated enzymes that can block viral replication. dsRNA replication is shown to occur in the cytoplasm for all dsRNA viruses. This dsRNA-specific mouse monoclonal antibody specifically recognizes dsRNA of more than 40-bp length. Clone rJ2 has been used to detect dsRNA intermediates of multiple types of viruses, including Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, and Polio virus in biological samples. It can be useful in understanding how anti-viral responses are initiated and what how viruses overcome and avoid these antiviral therapies.
Specificity
Clone rJ2 specifically recognizes double stranded RNA (dsRNA) of greater than 40 bp in length that is generated during the replication of positive sense genome viruses.
Immunogen
Double stranded RNA produced by positive sense genome viruses.
Application
Anti-dsRNA, clone rJ2, Cat. No. MABE1134, is a mouse monoclonal antibody that detects double stranded RNA (dsRNA) and has been tested for use in Immunocytochemistry and Immunofluorescence.
Immunofluorescence Analysis: A representative lot detected dsRNA in Immunofluorescent applications (Savidis, G., et. al. (2016). Cell Rep. 15(11):2323-30; Savidis, G., et. al. (2016). Cell Rep. 16(1):232-246).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Quality
Evaluated by Immunocytochemistry in Dengue virus infected A549 cells.
Immunocytochemistry Analysis: A 1:60 dilution of this antibody detected dsRNA in Dengue virus infected A549 cells.
Immunocytochemistry Analysis: A 1:60 dilution of this antibody detected dsRNA in Dengue virus infected A549 cells.
Physical form
Mouse monoclonal antibody in supernatant without preservatives.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Scotland E Farley et al.
Nature communications, 13(1), 3487-3487 (2022-06-18)
A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. Here, we map alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We find that SARS-CoV-2 rewires host lipid metabolism, significantly altering hundreds of lipid species to effectively
Amornrat O'Brien et al.
Virology, 556, 73-78 (2021-02-07)
The need to stem the current outbreak of SARS-CoV-2 responsible for COVID-19 is driving the search for inhibitors that will block coronavirus replication and pathogenesis. The coronavirus 3C-like protease (3CLpro) encoded in the replicase polyprotein is an attractive target for
Tomer M Yaron et al.
bioRxiv : the preprint server for biology (2020-08-21)
While vaccines are vital for preventing COVID-19 infections, it is critical to develop new therapies to treat patients who become infected. Pharmacological targeting of a host factor required for viral replication can suppress viral spread with a low probability of
Ismael Gomes et al.
Lancet Regional Health. Americas, 2, 100046-100046 (2021-09-07)
Neurological and other systemic complications occur in adults with severe COVID-19. Here we describe SARS-CoV-2 infection complicated by neuroinvasion in the post-mortem tissues of a child. We performed a complete autopsy of a 14-month-old child who died of COVID-19 pneumonitis.
Kim M Stegmann et al.
iScience, 25(5), 104293-104293 (2022-05-03)
The nucleoside analog N4-hydroxycytidine (NHC) is the active metabolite of the prodrug molnupiravir, which has been approved for the treatment of COVID-19. SARS-CoV-2 incorporates NHC into its RNA, resulting in defective virus genomes. Likewise, inhibitors of dihydroorotate dehydrogenase (DHODH) reduce
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Does the rJ2 antibody can recognize dsRNA from in an IVT reaction? J2 antibody can, so does rJ2?
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