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IPVH85R

Millipore

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Synonym(s):

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23

product name

Immobilon®-P Membrane, PVDF, 0.45 m, 8.5 cm x 10 m roll, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

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General description

Immobilon®-P transfer membrane is a hydrophobic polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is used for the transfer of proteins from a variety of gel matrices. Immobilon®-P transfer membrane is recommended for most western blotting, especially proteins greater than 20 kDa. This membrane displays improved handling characteristics and staining capabilities, a higher signal-to-noise ratio for enhanced sensitivities, and improved solvent resistance, when compared to nitrocellulose membrane.
Primary Binding Mechanisms: Electrostatic, hydrophobic,
Filter Code: IPVH

Application

Immobilon®-P Membrane, PVDF, 0.45 μm, 8.5 cm x 10 m roll has been used in western blot analysis and co-immunoprecipitation.

Linkage

Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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