This product can be resuspended in pH 8 Tris buffer, as mentioned on the Enzymatic Assay of Elastase webpage. For more details, please refer to the following link: https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-elastase#preparation
E0258
Elastase from porcine pancreas
Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)
Synonym(s):
Elastase from hog pancreas, Pancreatopeptidase E
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
254-453-6
MDL number:
EC Number:
Specific activity:
≥4.0 units/mg protein (biuret)
Biological source:
Porcine pancreas
Pricing and availability is not currently available.
biological source
Porcine pancreas
Quality Segment
type
Type IV
form
lyophilized powder
specific activity
≥4.0 units/mg protein (biuret)
composition
Protein, 50-90%
foreign activity
trypsin ≤50 BAEE units/mg protein
storage temp.
−20°C
General description
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Application
Elastase from porcine pancreas has been used:
- to treat vero cells to study its effects on syncytium formation
- as a positive control in protease assays
- as a component in RPMI 1640 to isolate human aortic smooth muscle cells (HASMCs) from the aortic tissue
Elastase from Sigma has been used to examine the extent of proteolytic degradation of BSA that is treated with hydroxyl radical. It has also been used to purify elastase-inhibitory lipid derivative from a cyanobacterium, Microcystis Ku2.
Elastase from porcine pancreas has been used in a study to assess the molecular bases for human leucocyte elastase inhibition. Elastase from porcine pancreas has also been used in a study to investigate the molecular cloning and expression of serum calcium-decreasing factor (caldecrin).
Biochem/physiol Actions
Elastase is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells. Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Elastase is a serine protease with broad specificity as it cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.
Packaging
Package size based on protein content
Physical form
Contains sodium carbonate.
Preparation Note
A further purification of Type III, E 0127, by affinity chromatography to reduce trypsin activity
Other Notes
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| description Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret) | description Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein | description Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues. | description lyophilized powder, ≥50 units/mg protein (Bradford) |
| biological source Porcine pancreas | biological source - | biological source - | biological source human leucocytes |
| specific activity ≥4.0 units/mg protein (biuret) | specific activity ≥4.0 units/mg protein | specific activity ≥25 units/mg protein | specific activity ≥50 units/mg protein (Bradford) |
| form lyophilized powder | form lyophilized powder | form lyophilized | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| foreign activity trypsin ≤50 BAEE units/mg protein | foreign activity - | foreign activity Chymotrypsin ≤0.2% | foreign activity - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Still not finding the right product?
Explore all of our products under Elastase from porcine pancreas
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
How does this product (elastase type IV) needs to be dissolved?
1 answer-
Helpful?
-