As indicated above, this product is soluble in distilled water to 10 mg/mL. Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
324682
Elastase, Porcine Pancreas, High Purity, Crystallized
Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.
Synonym(s):
Elastase, Porcine Pancreas, High Purity, Crystallized
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥25 units/mg protein
Pricing and availability is not currently available.
Quality Segment
description
Merck USA index - 14, 3535
form
lyophilized
specific activity
≥25 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
solubility
distilled water: 10 mg/mL
foreign activity
Chymotrypsin ≤0.2%
shipped in
ambient
storage temp.
−20°C
General description
Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.
Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
Physical form
Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.
Preparation Note
Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.
Other Notes
Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.
Mandl, I. 1962. Methods in Enzymol.5, 665.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
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| description Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues. | description Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein | description Type I, ≥4.0 units/mg protein | description Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans |
| specific activity ≥25 units/mg protein | specific activity ≥4.0 units/mg protein | specific activity ≥4.0 units/mg protein | specific activity ≥20 units/mg protein |
| form lyophilized | form lyophilized powder | form suspension | form lyophilized solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility distilled water: 10 mg/mL | solubility 200 mM Tris HCl buffer, pH 8.8: soluble 1.0 mg/mL, clear | solubility - | solubility - |
| shipped in ambient | shipped in - | shipped in - | shipped in ambient |
| storage condition desiccated, OK to freeze | storage condition - | storage condition - | storage condition OK to freeze |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
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How should the enzyme be reconstituted and stored?
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What type of elastase is 324682-250U? Is it type I, II, III, IV, or a mixture?
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This product is not currently tested for isoforms, so we cannot speculate on which isoforms are present or most prevalent in this product; however, based on a literature search, this product should be most consistent with isoforms from the pancreas.
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