CHO-K1 Cell Line from hamster
85051005, hamster ovary, Epithelial
Synonym(s):
CHO cell clone K1 Cells, CHOK1 Cells
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About This Item
UNSPSC Code:
41106514
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CHO-K1 Cell Line from hamster, 85051005, from hamster ovary
biological source
hamster ovary
growth mode
Adherent
karyotype
Hypodiploid, modal no. 20
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Chinese ovary
Cell Line Description
A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.
Application
CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.
Nutritional and gene expression studies
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25%Trypsin or Trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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