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biological source
mouse thymus
Quality Level
description
Mouse DBA/2 lymphoma L5178Y tk+/-
growth mode
Suspension
morphology
Small rounded cells
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
The L5178Y tk+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction.
Culture Medium
Fischer′s medium + 2mM Glutamine + 1% Sodium pyruvate + 10% heat-inactivated Horse Serum (HS)
Subculture Routine
Maintain cultures in logarithmic growth phase between 3 x 104 and 1 x 106 cells/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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