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SAE0159

Microbial Transglutaminase

lyophilized powder, ≥12 units/mg protein

Synonym(s):

Microbial Enzyme, Transglutaminase, Transglutaminase Enzyme

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About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥12 units/mg protein
Pricing and availability is not currently available.
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form

lyophilized powder

Quality Segment

200

specific activity

≥12 units/mg protein

shipped in

dry ice

storage temp.

−20°C

General description

Transglutaminases (TG) are a family of enzymes that catalyze isopeptide bond formation. This bond formation occurs between the γ-carboxyamide group of glutamine and various primary amines (primarily the ε-amino group of lysine). The resulting intermolecular or intramolecular cross-linking of transglutaminase is highly stable and shows high resistance to proteolytic degradation. The transglutaminase crosslinking activity support the formation of blood clots , skin and hair. On the other hand, TG is now being implicated in Celiac disease as well as in Huntington and Parkinson diseases. Historically, Microbial transglutaminase has been heavily used in the food industry. Microbial TG is often preferred for newer applications (such as site-specific protein modification and antibody drug conjugation) due to its small molecular weight and lack of calcium dependency when compared to the mammalian forms.

Application

Microbial Transglutaminase has been used for:
  • Protein cross-linking & site-specific labeling
  • Antibody Drug Conjugation
  • 3D bioprinting bioink preparation
  • Food related immunogenicity/pathogenicity related research

Features and Benefits

  • Small (~38kDa) and calcium independent enzyme
  • Highly purified lyophilized enzyme
  • Consistent and reproducible activity
  • Characterized for endotoxin content

Preparation Note

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices
Store the freeze-dried product at –20 °C. It is recommended to store the reconstituted protein in working aliquots at –20 °C to avoid repeated freeze/ thaw cycles.

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This Item
MAB3839-ICS1070616467
Microbial Transglutaminase lyophilized powder, ≥12 units/mg protein

Sigma-Aldrich

SAE0159

Microbial Transglutaminase

Anti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3 clone 4G3, from mouse

Sigma-Aldrich

MAB3839-I

Anti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3

Transglutaminase Assay Kit sufficient for assays in two 96-well plates

Sigma-Aldrich

CS1070

Transglutaminase Assay Kit

Transglutaminase 2 Inhibitor, ZDON A cell-permeable, peptide-based, irreversible inhibitor of transglutaminase 2 (TG2; IC50 = 150 nM for recombinant TG2). Reacts with the active site cysteine of TG2.

Sigma-Aldrich

616467

Transglutaminase 2 Inhibitor, ZDON

specific activity

≥12 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

lyophilized powder

form

-

form

-

form

powder

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

ambient

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

100

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  

  1. Hi, I have purchased Microbial Transglutaminase lyophilized powder, >12 units per mg (SAE0159-25UN). How can I prepare its stock? How much volume of solvent (for example PBS) should I add in the vial containing enzyme powder? 

    1 answer

    1. Reconstitute this product in 20 mM Tris-HCl containing 150 mM NaCl, in Molecular Biology grade water, pH 8 at 0.14 units per microliter. The unit per milligram activity is lot-specific and available in the product Certificate of Analysis. Further usage instructions are available in the product datasheet at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/339/389/sae0159pis-ms.pdf

      Please see the following link to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/US/en/product/sigma/sae0159*product-documentation

      Helpful?

  2. Bonjour, Est-il possible de savoir la masse totale d'enzyme dans le produit ? Les 25 units correspondent bien à l'activité de la poudre (mélange enzyme + excipients) ?

    1 answer

    1. The total mass of product in each vial varies from lot to lot due to variability in the protein content and the activity per milligram protein. The vial contents may be calculated based on information reported in the Certificate of Analysis. A review of recent lots reveals a range of 14 - 30% protein with an activity range of 14 -38 units per milligram protein.

      Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/sae0159#product-documentation

      Please see the following link to review the product data sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/339/389/sae0159pis-ms.pdf

      Helpful?

  3. こちらの製品は大腸菌由来のリコンビナントタンパクでしょうか？

    1 answer

    1. This is a cell culture fermentation product. The source organism is considered proprietary.

      Helpful?

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