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56312C

SAFC

Medium 199 Modified

with Earle′s Balanced Salts, with 0.68 mM L-glutamine, without sodium bicarbonate, dry powder, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Medium 199

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.71

description

for research or for further manufacturing use

Quality Level

500

form

dry powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: 100 mg/L
NaHCO3: no
Earle’s salts (5% CO2): yes
phenol red: 21.42 mg/L

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Components

Contains all L-form amino acids

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wuping Li et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 17(12), 2067-2077 (2009-07-16)
Recombinant adeno-associated virus (AAV) vectors expressing the cystic fibrosis transmembrane conductance regulator (CFTR) gene have been used to deliver CFTR to the airway epithelium of cystic fibrosis (CF) patients. However, no significant CFTR function has been demonstrated likely due to

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