M8884

Methyl Green

~85% purity, powder

• Synonym(s): Ethyl Green
• Linear Formula: C₂₇H₃₅BrClN₃ · ZnCl₂
• CAS Number: 7114-03-6
• Molecular Weight: 653.24
• Colour Index Number: 42590
• Beilstein: 3914106
• EC Number: 230-415-4
• MDL number: MFCD00151094
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897293
• NACRES: NA.47

Properties

• product name: Methyl Green, zinc chloride salt, ~85%
• Quality Level: 200
• Assay: ~85%
• form: powder
• composition: Dye Content, ≥83%
• technique(s): titration: suitable
• color: red to very dark red
• pH: 0.2-1.8, yellow to blue
• mp: >300 °C (lit.)
• solubility: water: 1 mg/mL
• εmax: >11100.00 at 417-425 nm in water at 0.005 g/L; >16000 at 311-319 nm in water at 0.005 g/L; >60000 at 626-634 nm in water at 0.005 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Description

General description

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Application

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Quality

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.

Analysis Note

Dye content based on molecular weight of monobromo-monochloro ZnCl₂ salt (653.2).

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H314,H335,H411
• Precautionary Statements: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves