MABD34

Anti-Werner′s syndrome helicase (WRN) Antibody, clone 20A11.1

clone 20A11.1, from mouse

• Synonym(s): Werner syndrome ATP-dependent helicase, DNA helicase, RecQ-like type 3, RecQ3, Exonuclease WRN, RecQ protein-like 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 20A11.1, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000544
• UniProt accession no.: Q14191
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WRN(7486)

Description

General description

The Werner’s syndrome helicase (RecQ-like type 3; RecQ3; Exonuclease WRN) is a ubiquitously expressed nuclear protein belonging to the RecQ family. It functions as a helicase and exonuclease enzyme, and plays a crucial role in the formation of DNA replication centers. This protein is also involved in DNA repair and recombination, and may be involved in transcriptional events mediated by RNA polymerase II. It is regulated by phosphorylation and is targeted by the DNA-dependent protein kinase enzyme. Disrupted expression of the gene encoding the Werner’s syndrome helicase protein often results in the production of truncated Werner’s syndrome helicase proteins. Over 50 gene mutations may contribute to this effect—and lead to the development of Werner syndrome, a condition characterized by accelerated aging and early onset of age-related conditions such as cancer, atherosclerosis, and osteoporosis.

Immunogen

GST-tagged recombinant protein corresponding to human WRN.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-WRN Antibody, clone 20A11.1 (Mouse Monoclonal Antibody) validated in WB to detect WRN clone 20A11.1 also known as Werner syndrome ATP-dependent helicase, RecQ3, Exonuclease WRN.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Werner′s syndrome helicase (WRN) in 10 µg of HEK293 cell lysate.

Target description

~162 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable