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Key Documents

AB5889

Sigma-Aldrich

Anti-Capsaicin Receptor Antibody, NT

serum, Chemicon®

Synonym(s):

Vanilloid Receptor 1, VR1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

primate, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TRPV1(7442)

Specificity

Capsaicin Receptor (VR1), N-terminal.

SPECIES REACTIVITIES:

Rat, mouse and primate. Other species have not been tested.

Immunogen

Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of rat VR1.

Application

Immunoblotting: 1:1,000

Immunohistochemistry: 1:1,000

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5889

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5889. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

IMMUNOHISTOCHEMISTRY

VR1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.

WESTERN BLOT

For details see Guo, A., et al., Eur J Neurosci (1999) 11:946-958.
Research Category
Neuroscience
Research Sub Category
Neuroinflammation & Pain

Neurotransmitters & Receptors
This Anti-Capsaicin Receptor Antibody, N-terminus is validated for use in WB, IH for the detection of Capsaicin Receptor.

Physical form

Liquid. Contains 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

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