HPLC Analysis of Norgestrel Enantiomers on Astec^® CYCLOBOND II

column

CYCLOBOND II, 25 cm x 4.6 mm I.D., 5 μm particles (41020AST)

mobile phase

[A] water; [B] acetonitrile; (70:30, A:B)

flow rate

0.8 mL/min

column temp.

22 °C

detector

UV, 254 nm

injection

1 μL

sample

norgestrel, 1 mg/mL in methanol