跳转至内容
Merck
  • Novel role of the serine protease inhibitor elafin in gluten-related disorders.

Novel role of the serine protease inhibitor elafin in gluten-related disorders.

The American journal of gastroenterology (2014-04-09)
Heather J Galipeau, Michelle Wiepjes, Jean-Paul Motta, Jessica D Schulz, Jennifer Jury, Jane M Natividad, Ines Pinto-Sanchez, Daniel Sinclair, Perrine Rousset, Rebeca Martin-Rosique, Luis Bermudez-Humaran, Jean Christophe Leroux, Joseph A Murray, Edgardo Smecuol, Julio C Bai, Nathalie Vergnolle, Philippe Langella, Elena F Verdu
摘要

Elafin, an endogenous serine protease inhibitor, modulates colonic inflammation. We investigated the role of elafin in celiac disease (CD) using human small intestinal tissues and in vitro assays of gliadin deamidation. We also investigated the potential beneficial effects of elafin in a mouse model of gluten sensitivity. Epithelial elafin expression in the small intestine of patients with active CD, treated CD, and controls without CD was determined by immunofluorescence. Interaction of elafin with human tissue transglutaminase-2 (TG-2) was investigated in vitro. The 33-mer peptide, a highly immunogenic gliadin peptide, was incubated with TG-2 and elafin at different concentrations. The degree of deamidation of the 33-mer peptide was analyzed by liquid chromatography-mass spectrometry. Elafin was delivered to the intestine of gluten-sensitive mice using a recombinant Lactococcus lactis vector. Small intestinal barrier function, inflammation, proteolytic activity, and zonula occludens-1 (ZO-1) expression were assessed. Elafin expression in the small intestinal epithelium was lower in patients with active CD compared with control patients. In vitro, elafin significantly slowed the kinetics of the deamidation of the 33-mer peptide to its more immunogenic form. Treatment of gluten-sensitive mice with elafin delivered by the L. lactis vector normalized inflammation, improved permeability, and maintained ZO-1 expression. The decreased elafin expression in the small intestine of patients with active CD, the reduction of 33-mer peptide deamidation by elafin, coupled to the barrier enhancing and anti-inflammatory effects observed in gluten-sensitive mice, suggest that this molecule may have pathophysiological and therapeutic importance in gluten-related disorders.

材料
货号
品牌
产品描述

Sigma-Aldrich
转谷氨酰胺酶 来源于天竺鼠肝脏, lyophilized powder, ≥1.5 units/mg protein
价格与库存信息目前不能提供
Sigma-Aldrich
醇溶蛋白 来源于小麦
价格与库存信息目前不能提供
Sigma-Aldrich
人Trappin-2 / Elafin  ELISA试剂盒, for serum, plasma, cell culture supernatant and urine
价格与库存信息目前不能提供