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應用
Sigma的该产品已用于证明组织转谷氨酰胺酶（tTG）选择性地使谷蛋白肽脱酰胺，这导致强烈增强的T细胞刺激活性。其还可用于评估对 A-麦醇溶蛋白肽的免疫应答。此外，它已用于证明 tTG 选择性地修饰在乳糜泻中由肠源性 T细胞识别的麦醇溶蛋白肽。
转谷氨酰胺酶已用于一项可改善可量化分析研究，以充分表征转谷氨酰胺酶在亨廷顿氏′s病和阿尔茨海默′s病等疾病中的作用。转谷氨酰胺酶还用于研究转谷氨酰胺酶活性的非放射性斑点印迹试验。
生化／生理作用
来自豚鼠肝脏的转谷氨酰胺酶由691个氨基酸残基的单一多肽链组成。它有六个潜在的糖基化位点（Asn-X-Ser或Asn-X-Thr），但它没有糖基化。分子量约为76.6kDa。它是钙依赖性的，有几个钙结合位点。在钙存在下，碘乙酰胺和N-乙基马来酰亚胺抑制该酶的活性。它催化小分子量胺类结合到蛋白质的γ-谷氨酰胺位点。在不存在小分子量胺类的情况下，它催化蛋白质的交联，导致 γ-谷氨酰基-ε-赖氨酸侧链肽的形成。肝转谷氨酰胺酶是非酶原的酶。
單位定義
一个单元，在pH6.0，37℃下，每分钟将催化Nα-Z-Gln-Gly和羟胺形成1.0 μmole的异羟肟酸酯。（L-谷氨酸γ-单羟肟酸酯为参考标准。）
外觀
含 Tris 和二硫赤藓醇的冻干粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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