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Merck
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50859-U

Supelco

Ascentis® Express 90 Å F5 (2 μm) HPLC Columns

L × I.D. 5 cm × 2.1 mm UHPLC Column

别名:

Core-shell (SPP) Fused Core PFP HPLC column

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

product name

Ascentis® Express F5, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 5 cm × 2.1 mm

材料

stainless steel column

品質等級

100

agency

suitable for USP L43

產品線

Ascentis®

特點

endcapped

製造商／商標名

Ascentis®

包裝

1 ea of

參數

1000 bar max. pressure (14500 psi)
60 °C temp. range

技術

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

長度 × 內徑

5 cm × 2.1 mm

表面積

120 m2/g

雜質

<5 ppm metals

基質

Fused-Core particle platform
superficially porous particle

基質活性組

PFP (pentafluorophenyl) phase

粒徑

2 μm

孔徑

90 Å pore size

工作pH值

2-8

應用

food and beverages

分離技術

reversed phase

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相关类别

一般說明

Ascentis® Express 2.0 μm F5是基于Fused-Core®颗粒设计的快速UHPLC柱。这种颗粒设计在0.4 μm厚的多孔壳中的扩散路径较浅，且总体粒径小至2 μm，因此柱效很高。Ascentis® Express 2.0 μm F5的键合封端的二甲基五氟苯基丙基固定相由于存在负电性氟而可提供含缺电子苯环的稳定的反相填充。除了形成π-π相互作用和温和的空间相互作用外，F5相还通过极性相互作用保留化合物。F5相具有极性和非极性特性，可表现出双模式保留行为，有时会随流动相中乙腈含量的变化而产生“ U形”保留，在ACN浓度低和高的情况下保留都会增加（ 反相和HILIC保留模式）。Ascentis® Express 2.0 μm F5可作为C18选择性的补充，对碱性、酸性或中性化合物进行分析。

應用

使用Ascentis® Express F5（2 μm）对霉菌毒素进行UHPLC/MS分析

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法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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分析证书(COA)

Lot/Batch Number
USSDS001427
USSDS001426
USSDS001425
USSDS001424
USSDS001423

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
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Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Small Molecule HPLC

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Small Molecule HPLC

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