ZRB1415

Anti-Nucleolin Antibody, clone 5D13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Protein C23
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 76.61 kDa; observed mol wt ~110 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: P19338
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NCL(4691)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 5D13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nucleolin. It targets an epitope within 16 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Nucleolin.

應用

Anti-Nucleolin, clone 5D13 ZooMAb, Cat. No. ZRB1415, is a recombinant Rabbit monoclonal antibody that targets Nucleolin and is tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nucleolin in A431 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nucleolin in HeLa, HUVEC, as A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nucleolin in human small intestine tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Nucleolin (UniProt: P19338; also known as Protein C23) is encoded by the NCL gene (Gene ID: 4691) in human. Nucleolin is a multifunctional phosphoprotein that is ubiquitously distributed in the nucleolus, nucleus and cytoplasm of the cell. It is one of the most abundant non-ribosomal proteins that accounts for about 10% of the protein content within the nucleolus. It is found associated with intra-nucleolar chromatin and pre-ribosomal particles. It plays important roles in various steps of ribosomal synthesis, including the transcription of rDNA repeats, the modification and processing of pre-rRNA, the assembly of pre-ribosomal particles and nuclear-cytoplasmic transport of ribosomal proteins and subunits. Nucleolin contains three structural and multifunctional domains: the N-terminal domain contains several acidic stretches; two to four RNA-binding domains (RNA recognition motifs or RRM) in the central region; and a glycine/arginine-rich domain (GAR) domain at the C-terminus. Nucleolin is shown to be highly phosphorylated. It undergoes extensive phosphorylation by casein kinase 2 at interphase and by CDC2 during mitosis. It has been reported that Nucleolin phosphorylation at interphase is correlated with active rRNA transcription. Although the mammalian Nucleolin has a predicted molecular weight of about 77 kDa, its apparent molecular weight lies between 100 and 110 kDa due to highly phosphorylated amino acids in the N-terminal domain. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tajrishi, MM et al., (2011). Commun. Integr. Biol. 4(3); 267-275).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

0.03 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2