推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1G5, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt ~61.52 kDa
observed mol wt ~59 kDa
物種活性
mouse, rat, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1G5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Transcription Factor E3 (TFE3). It targets an epitope within the C-terminal half.
免疫原
His-tagged recombinant fragment corresponding to 143 amino acids from the C-terminal half of human Transcription factor E3 (TFE3).
應用
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFE3 in NIH3T3 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TFE3 in HeLa and C6 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Recombinant TFE3 Protein with a KD of 1.6 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TFE3 in human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TFE3 in PC3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TFE3 in NIH3T3 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TFE3 in HeLa and C6 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Recombinant TFE3 Protein with a KD of 1.6 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TFE3 in human testis tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TFE3 in PC3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TFE3, clone 1G5 ZooMAb®, Cat. No. ZRB1272, is a recombinant Rabbit monoclonal antibody that specifically targets TFE3 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
標靶描述
Transcription factor E3 (UniProt: P19532; also known as Class E basic helix-loop-helix protein 33, bHLHe33, TFE3) is encoded by the TFE3 (also known as BHLHE33) gene (Gene ID: 7030) in human. TFE3 is a ubiquitously expressed transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. It specifically recognizes and binds E-box sequences (5′-CANNTG-3′) and its efficient DNA-binding requires homodimerization or heterodimerization with another member of the MiT/TFE family, such as TFEB or MITF. TFE3 is reported to be involved in the cellular response to amino acid availability by acting downstream of MTOR. In the presence of nutrients, it undergoes phosphorylation by mTOR that promotes its cytosolic retention and subsequent inactivation. However, under conditions of starvation or lysosomal stress, inhibition of mTOR induces TFE3 dephosphorylation that results in its nuclear localization and transcription factor activity. TFE3 is also reported to act as a positive regulator of browning of adipose tissue by promoting expression of target genes. It also plays a role in maintenance of the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB. Its mTOR-dependent nuclear exclusion promotes exit of cells from pluripotency. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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