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Merck

TRUEDEXS

Sigma-Aldrich

TrueGel3D聚合物

Modified for slow gelling (SLO-DEXTRAN), allow cell recovery

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71

技术

cell attachment: suitable

运输

dry ice

储存温度

−70°C

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一般描述

Dextrose polymers are functionalized with thiol-reactive groups, which react at a slower rate with thiol groups of crosslinkers (CD/PEG crosslinkers) to form stable hydrogels. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of homogenous gel formation. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

应用

SLO-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

特点和优势

  • Contains dextrose polymer functionalized with thiol reactive groups for slow gelation
  • Degradable polymer

组分

TRUEDEXS contains SLO-DEXTRAN solution in phosphate buffer, TrueGel3D thioglycerol and water

注意

Avoid frequent freeze-thaw cycles and store SLO-DEXTRAN in small portions

象形图

Corrosion

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

储存分类代码

8A - Combustible corrosive hazardous materials


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

Troubleshoot TrueGel3D™ hydrogel formation and cell viability issues with RGD-degradable polymer and CD cell-degradable crosslinker.

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实验方案

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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