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运输
dry ice
储存温度
−70°C
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一般描述
The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on PEG non cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.
应用
TRUE5-1KT is preferred over dextran-based gels for long-term culture of cells which producing carbohydrate degrading enzymes. It is used to culture spheroids which proliferate but do not attach to hydrogel. PVA-PEG hydrogel kits are also used to immobilize cells or specimens for imaging.
特点和优势
- Fast gelling (takes few seconds to minutes after addition of crosslinker)
- Flexibility in modulating gel stiffness
- Does not allow cell migration
- Cell inert hydrogel (if not customized with RGD peptide)
- Cells cannot be recovered
组分
TRUE5-1KT contains FAST-PVA (polymer) in solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.
注意
Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store Mal-PVA in small portions to avoid frequent freeze-thaw cycles.
警示用语：
Warning
危险声明
预防措施声明
危险分类
Met. Corr. 1
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and several other diseases.
TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions
TTrueGel3D™是一种用于3D细胞培养的合成水凝胶平台，可模拟天然细胞外环境以获得更符合生理的培养条件
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