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Merck
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Key Documents

T1428

Sigma-Aldrich

脱铁-转铁蛋白 牛

BioReagent, suitable for cell culture, ≥98%

别名:

Human serotransferrin

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.83

生物源

bovine

產品線

BioReagent

化驗

≥98%

形狀

powder

技術

cell culture | mammalian: suitable

雜質

iron, essentially free

溶解度

H2O: 10 mg/mL

UniProt登錄號

儲存溫度

2-8°C

基因資訊

bovine ... TF(280705)

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一般說明

脱铁转铁蛋白是一种无铁形式的转铁蛋白,存在于循环系统中。

應用

所有细胞都需要铁才能从其环境中适当吸收氧气。 转铁蛋白是一种从血清中分离出来的蛋白质,可引起铁从培养基中摄取并转运到细胞中。 这允许适当的氧气吸收,并且还刺激与生长相关的酶活性。 铁饱和转铁蛋白(全转铁蛋白)在释放铁后不断循环,变成缺铁转铁蛋白(脱辅基转铁蛋白)。 选择使用哪种形式取决于培养条件。 在诸如含有高水平铁盐的Dulbecco′s改良的Eagle′s培养基/Ham′s营养混合物F12(DMEM/F12)的培养基中,优选使用脱铁转铁蛋白。
牛载脂蛋白转铁蛋白已用作以下材料的组分:
  • 牛乳腺上皮细胞培养的基础培养基
  • 精原干细胞培养的最低必需培养基α(MEM-α)
  • 间充质干细胞培养的诱导培养基

生化/生理作用

脱铁转铁蛋白(apoTf)有助于防止铁在组织中积聚,特别是在非输血依赖型β地中海贫血中。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Monique P Gelderman et al.
Haematologica, 100(5), 611-622 (2015-01-27)
Intermediate beta-thalassemia has a broad spectrum of sequelae and affected subjects may require occasional blood transfusions over their lifetime to correct anemia. Iron overload in intermediate beta-thalassemia results from a paradoxical intestinal absorption, iron release from macrophages and hepatocytes, and
Keita Abe et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 154-156 (2008-04-05)
We have investigated the mechanism of manganese ion uptake by apo-transferrin using a capillary electrophoresis method, and obtained clear evidence that oxidation state +3 and the binuclear unit of a manganese chelate are essential factors for the facile uptake by
Einar Vargas-Bello-Pérez et al.
Animal nutrition (Zhongguo xu mu shou yi xue hui), 5(2), 202-208 (2019-06-14)
The objective of this study was to determine how cytosolic triacylglycerols (TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids (FA). This objective was accomplished by addition of different FA to
Shanshan Li et al.
Journal of animal science and biotechnology, 7, 44-44 (2016-08-06)
The objective of this study was to study how changing the ratio of Lys to Thr, Lys to His, and Lys to Val affects the expression of lipogenic genes and microRNA (miRNA) in bovine mammary epithelial cells. Triplicate cultures with
Jia Wang et al.
Stem cell research & therapy, 10(1), 227-227 (2019-07-31)
High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still

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