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Merck
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文件

T0377

Sigma-Aldrich

三(羟甲基)甲基甘氨酸

≥99% (titration)

别名:

N-三(羟甲基)甲基甘氨酸

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About This Item

线性分子式:
(HOCH2)3CNHCH2CO2H
CAS号:
5704-04-1
分子量:
179.17
Beilstein:
1937804
EC號碼:
227-193-6
MDL號碼:
MFCD00004277
分類程式碼代碼:
12161700
PubChem物質ID:
24899884
NACRES:
NA.25

化驗

≥99% (titration)

形狀

crystalline powder

有用的pH值範圍

7.4-8.8

pKa (25 °C)

8.1

mp

187 °C

溶解度

water: 0.25 g/mL, clear, colorless

應用

diagnostic assay manufacturing

SMILES 字串

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI 密鑰

SEQKRHFRPICQDD-UHFFFAOYSA-N

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一般說明

Tricine 是最常用的电泳缓冲液，也可用于细胞颗粒重悬。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）系统相比，在丙烯酰胺浓度较低的情况下，Tricine 起到尾随离子的作用，有助于小蛋白质的分离。它的负电荷比甘氨酸低，这有助于它更快地迁移。Tricine 具有高离子强度，可以增加离子运动，减少蛋白质运动。这反过来又导致低分子量蛋白质在较低百分比的丙烯酰胺凝胶中分离。

應用

Tricine 已用于：
  • 防止赫氏颗石藻培养物高压灭菌过程中的盐沉淀
  • 作为缓冲液 A 组分，用于均质化秀丽隐杆线虫、果蝇和植物等样品
  • 作为新鲜检测缓冲液成分，放射免疫法测定血清褪黑素

用于分离低分子量的肽的缓冲剂组分。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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乙二胺-N,N′-二乙酸 ≥98%

Sigma-Aldrich

158186

乙二胺-N,N′-二乙酸

EPPS ≥99.5% (titration)

Sigma-Aldrich

E9502

EPPS

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Encyclopedia of Dairy Sciences (2011)
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The avian pineal gland, apart from the hypothalamic master clock (suprachiasmatic nuclei, SCN) and retina, functions as an independent circadian oscillator, receiving external photic cues that it translates into the rhythmical synthesis of melatonin, a biochemical signal of darkness. Functional
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Nature protocols, 8(1), 43-51 (2012-12-12)
The lipid droplet (LD) is a cell organelle that has been linked to human metabolic syndromes and that can be exploited for the development of biofuels. The isolation of LDs is crucial for carrying out morphological and biochemical studies of
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Biofouling, 29(1), 77-85 (2012-12-06)
The freshwater zebra mussel (Dreissena polymorpha) is a notorious biofouling organism. It adheres to a variety of substrata underwater by means of a proteinaceous structure called the byssus, which consists of a number of threads with adhesive plaques at the
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实验方案

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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