跳转至内容
Merck
所有图片(1)

Key Documents

SRP0234

Sigma-Aldrich

PI3 kinase [p110a(E545K)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

别名:

PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352200
NACRES:
NA.77

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥55% (SDS-PAGE)

形狀

aqueous solution

比活性

≥800 pmol/min-μg

分子量

129 kDa
88 kDa

包裝

pkg of 20 μg

濃度

0.56 mg/mL

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... PIK3CA(5290)

一般說明

Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门