SRP0211

PTP-D2/PTPN14 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 别名: Cytoskeletal-associated protein tyrosine phosphatase, PTP36, PTPN14, Pez, Protein tyrosine phosphatase, non-receptor type 14
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 61 kDa
• 包裝: pkg of 20 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_005401
• UniProt登錄號: Q15678
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PTPN14(5784)

说明

一般說明

Catalytic domain, (amino acids 817-1188), (Gene bank accession number NM_005401) with N-terminal GST tag, MW = 61 kDa, expressed in an E. coli expression system.

應用

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

單位定義

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.