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生物源
human
重組細胞
expressed in E. coli
化驗
≥90% (SDS-PAGE)
形狀
aqueous solution
分子量
28 kDa
包裝
pkg of 100 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
>0.02 mg/mL
NCBI登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... BCL2L1(598)
一般說明
BCL2L1 (B-cell lymphoma 2 like 1) gene codes for the anti-apoptotic protein called Bcl-xL (B-cell lymphoma-extra large) and its isoform Bcl-xS. Bcl-xS functions as an antagonist of Bcl-xL. This gene is localized to human chromosome 20q11.21.
應用
Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.
生化／生理作用
BCL2L1 (B-cell lymphoma 2 like 1) or Bcl-xS functions as a dominant negative regulator of the anti-apoptotic isoform Bcl-xL. Bcl-xL is involved in modulating anti-apoptotic capacity of chemotherapy-resistant tumors. This protein is highly expressed in michigan cancer foundation 7 (MCF7) human breast carcinoma cells, and studies show that overexpression of Bcl-xS in these MCF-7 cells results in heightened sensitivity to chemotherapy-induced apoptosis by agents such as VP-16 or taxol.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 2
Bcl-xL mediates therapeutic resistance of a mesenchymal breast cancer cell subpopulation.
Keitel U, et al.
Oncotarget, 5(23), 11778-11791 (2014)
BCL-XL mediates the strong selective advantage of a 20q11.21 amplification commonly found in human embryonic stem cell cultures.
Avery S, et al.
Stem Cell Reports, 1(5), 379-386 (2013)
Gain of 20q11.21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL.
Nguyen HT, et al.
Molecular Human Reproduction, 20(2), 168-177 (2014)
Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis.
Sumantran VN, et al.
Cancer Research, 55(12), 2507-2510 (1995)
Francis McCoy et al.
Methods in molecular biology (Clifton, N.J.), 1133, 119-140 (2014-02-26)
The study of apoptosis and caspases has advanced greatly over recent decades. Studies conducted in the Xenopus laevis egg extract and oocyte model system have significantly contributed to these advances. Twenty years ago, Newmeyer and colleagues first showed that the
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