SRE0019

三磷酸腺苷双磷酸酶 来源于马铃薯

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

• 别名: 腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶
• CAS号: 9000-95-7
• EC 号: 3.6.1.5 (BRENDA, IUBMB)
• EC號碼: 232-569-8
• MDL號碼: MFCD00130542
• 分類程式碼代碼: 12352204
• NACRES: NA.32

属性

• 品質等級: 400
• 種類: Grade VII
• 形狀: lyophilized powder
• ATPase activity: ≥200 units/mg protein
• secondary activity: ≥50 % of base activity ADPase
• 成份: Protein, 25-60%
• 溶解度: H₂O: soluble 1.0 mg/mL
• 應用: diagnostic assay manufacturing
• 異物活動: Acid Phosphatase ≤2% of base activity
• 儲存溫度: −20°C

说明

應用

不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+P_i → AMP+2P_i。

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1.[1] Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation[2].

生化／生理作用

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis[3]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

單位定義

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

外觀

Partially purified, lyophilized powder containing potassium succinate buffer salts.

準備報告

按 ATP 酶单位销售。

其他說明

该等级具有低 ATP 酶/ADP 酶比率。

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable