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Merck
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SHC003V

Sigma-Aldrich

MISSION®

Green fluorescent protein marker to monitor transduction efficiency

别名:

MISSION®, MISSION® TurboGFP™ Control Transduction Particles

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About This Item

UNSPSC代码:
41106609
NACRES:
NA.51

质量水平

100

产品线

MISSION®

浓度

≥1x106 VP/ml (via p24 assay)

技术

capture ELISA: 106 TU/mL using p24

运输

dry ice

储存温度

−70°C

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相关类别

一般描述

当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

应用

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

法律信息

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.

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产品编号
说明
价格

SHC002

任务 ® pLKO.1-puro 非哺乳动物 shRNA 对照质粒 DNA, Targets no known mammalian genes

SHC001V

MISSION®pLKO.1-puro空载体对照转导颗粒, Contains no shRNA insert

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ 阳性对照质粒 DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION®, Targets no known mammalian genes

SHC004

MISSION® 对照载体, shRNA sequence targeting tGFP

SHC004V

MISSION® TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

ExpressMag® Super Magnetic Kit, Increases transduction efficiency

SHM02

ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency

SHC012V

MISSION®, Contains a gene encoding TagRFP

SHC014V

MISSION®, Contains a gene encoding TurboGFP, under the control of the UbC promoter

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
06072112MN
09041810MN

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Esperanza Martín-Sánchez et al.
PloS one, 9(11), e112148-e112148 (2014-11-12)
Currently, there is no efficient therapy for patients with peripheral T cell lymphoma (PTCL). The Proviral Integration site of Moloney murine leukemia virus (PIM) kinases are important mediators of cell survival. We aimed to determine the therapeutic value of PIM
A high-content image-based method for quantitatively studying context-dependent cell population dynamics.
Garvey CM
Scientific Reports, 6, 29752-29752 (2016)
Ruoxiang Wang et al.
The Prostate, 80(3), 274-283 (2019-12-18)
We previously determined that cancer-stromal interaction was a direct route to tumor cell heterogeneity progression, since cancer-stromal cell fusion in coculture resulted in the creation of heterogeneous clones of fusion hybrid progeny. In this report, we modified the cancer-stromal coculture
Two-Photon Microscopy Analysis of Gold Nanoparticle Uptake in 3D Cell Spheroids.
Rane TD and Armani AM
PLoS ONE, 11(12) (2016)
Chi-Li Chiu et al.
Scientific reports, 6, 22435-22435 (2016-03-05)
The androgen receptor (AR) pathway plays a central role in prostate cancer (PCa) growth and progression and is a validated therapeutic target. In response to ligand binding AR translocates to the nucleus, though the molecular mechanism is not well understood.
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商品

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

实验方案

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

慢病毒转导实验方案

如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导，以实现稳定的长期沉默和表型变化的详细操作步骤。

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

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