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Merck

SHM01

Sigma-Aldrich

ExpressMag® Super Magnetic Kit

Increases transduction efficiency

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About This Item

UNSPSC代码:
41106609

产品线

MISSION®

保质期

≥2 yr at ~4 °C

储存温度

2-8°C

一般描述

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

应用

MISSION® ExpressMag® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.

注意

Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.
Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

法律信息

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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产品编号
说明
化学品安全说明书

  • SHM03MISSION® ExpressMag® Beads, Increases transduction efficiency化学品安全说明书

  • SHM04MISSION® ExpressMag® Super Magnetic Plate, Increases transduction efficiency化学品安全说明书

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

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实验方案

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

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