推荐产品
一般說明
面向全基因组扩增（WGA）的 SeqPlex™ Enhanced DNA 扩增试剂盒专用于对极小量或降解/高度片段化的 DNA 进行下一代测序（NGS）。 染色质免疫沉淀（ChIP）或福尔马林固定的石蜡包埋组织样品（FFPE）的DNA含量往往无法满足成功制备下一代测序文库的需求。通过 SeqPlex 试剂盒可对这类 DNA 以及其他用于 NGS 的小量/高度片段化 DNA 样品进行预扩增。 该试剂盒是 WGA 产品线的延伸，旨在集成到 Illumina®、SOLiD™ 或 454 测序工作流程中。
應用
SeqPlex™ DNA 扩增试剂盒已用于全基因组扩增。
特點和優勢
- 采用随机引物技术扩增片段化DNA，如ChIP或FFPE
- 有助于对低至100 pg的ChIP DNA进行测序
- 采用增强型引物，可获得完整的基因组覆盖、最低的序列偏差、引物清除以及适用于二代测序的扩增子大小
- 可兼容用于二代测序的Illumina®、SOLiD™或454文库制备
其他說明
SEQXE-500RXN 是按需生产。获取更多信息，请发邮件至[email protected] 联系技术服务。
法律資訊
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书
- Library Preparation Buffer
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR).
Kunnev D, et al.
Journal of biological methods, 2(4) (2015)
Xiang Yu et al.
Genome research, 27(7), 1238-1249 (2017-04-08)
Type II topoisomerases orchestrate proper DNA topology, and they are the targets of anti-cancer drugs that cause treatment-related leukemias with balanced translocations. Here, we develop a high-throughput sequencing technology to define TOP2 cleavage sites at single-base precision, and use the
Syuzo Kaneko et al.
Cancers, 13(9) (2021-05-01)
Although chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) using formalin-fixed paraffin-embedded tissue (FFPE) has been reported, it remained elusive whether they retained accurate transcription factor binding. Here, we developed a method to identify the binding sites of the insulator transcription factor
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
实验方案
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门