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主要文件

SAB4504721

Sigma-Aldrich

抗-磷酸-p47 phox(pSer345) 兔抗

affinity isolated antibody

别名:

Anti-CGD1, Anti-NCF1A, Anti-NOXO2, Anti-SH3PXD1A, Anti-p47phox

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen 44 kDa

物種活性

rat, human, mouse

濃度

~1 mg/mL

技術

ELISA: 1:1000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

phosphorylation (pSer345)

基因資訊

human ... NCF1(653361)

一般說明

中性粒细胞胞质因子1(NCF1),也被称为p47phox基因,大小为15kb,具有11个外显子,定位于人类染色体7q11.23上。该基因可编码一个390个氨基酸的蛋白质,该蛋白质是NADPH氧化酶复合物的一个新型组成部分。

免疫原

针对从人p47 phox Ser345的磷酸化位点周围衍生的合成肽产生抗血清。

免疫原范围:311-360

生化/生理作用

中性粒细胞胞浆因子1(NCF1)在高氧条件下刺激产生活性氧(ROS)。上述过程可被NCF1抑制剂二苯基碘化物(DPI)和罗布麻宁抑制。对该基因的突变会导致一种罕见的先天性免疫缺陷,称为慢性肉芽肿性疾病(CGD)。NCF1基因删除是NADPH氧化酶复合体的重要组成部分,与氧化应激的产生有交,会导致Williams综合征。它还可作用血管硬度的修饰剂。

特點和優勢

您可完全放心的评估我们的抗体。如果抗体在您的应用中没有发挥效果,我们将给予您全额赔偿或更换抗体。了解更多。

外觀

兔IgG,溶于磷酸盐缓冲盐水(不含Mg2+和Ca2+),pH7.4,150mM NaCl,0.02%叠氮化钠和50%甘油中。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途,或人类或动物任何类型的消费或使用。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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Astghik Hayrapetyan et al.
Biochimica et biophysica acta, 1832(10), 1662-1672 (2013-05-22)
Chronic granulomatous disease (CGD) is a rare congenital disorder in which phagocytes cannot generate superoxide (O2(-)) and other microbicidal oxidants due to mutations in one of the five components of the O2(-)-generating NADPH oxidase complex. The most common autosomal subtype
Beth A Kozel et al.
Hypertension (Dallas, Tex. : 1979), 63(1), 74-79 (2013-10-16)
Williams syndrome is caused by the deletion of 26 to 28 genes, including elastin, on human chromosome 7. Elastin insufficiency leads to the cardiovascular hallmarks of this condition, namely focal stenosis and hypertension. Extrapolation from the Eln(+/-) mouse suggests that
Lingping Zhang et al.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 32(1), 59-62 (2016-01-06)
To observe the effects of NADPH oxidase inhibitor diphenylene iodonium (DPI) and apocynin on the generation of reactive oxygen species (ROS) induced by p47phox and the mechanism of p47phox-induced ROS production under hyperoxic conditions. Peripheral blood mononuclear cells (PBMCs) were
U Francke et al.
American journal of human genetics, 47(3), 483-492 (1990-09-01)
Chronic granulomatous disease (CGD) is a heterogeneous group of inherited disorders of impaired superoxide production in phagocytes. The most common X-linked recessive form involves the CYBB locus in band Xp21.1 that encodes the membrane-bound beta subunit of the cytochrome b558
Trishan Vaikunthanathan et al.
EBioMedicine, 95, 104778-104778 (2023-09-02)
Dysregulated inflammatory responses and oxidative stress are key pathogenic drivers of chronic inflammatory diseases such as liver cirrhosis (LC). Regulatory T cells (Tregs) are essential to prevent excessive immune activation and maintain tissue homeostasis. While inflammatory cues are well known

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