SAB4200740

Anti-Neurofilament 160 antibody, Mouse monoclonal

clone NN18, purified from hybridoma cell culture

• 别名: Anti-160 kDa neurofilament protein, Anti-NF-M, Anti-Neurofilament 3, Anti-Neurofilament triplet M protein
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: NN18, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~160 kDa
• 濃度: ~1.0 mg/mL
• 技術: immunoblotting: 0.3-0.6 μg/mL using rat brain extract; immunofluorescence: 10-20 μg/mL using human bone marrow neuroblast SHSY5Y cell line; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain sections
• 同型: IgG1
• UniProt登錄號: P07197
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified

说明

一般說明

Anti-Neurofilament 160 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the NN18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with neurofilaments purified from pig spinal cord. Neurofilaments (NFs) belong to the intermediate filament (IF) family and are expressed mainly in cells or tissues of neuronal origin. Three major NFs subunits were discovered: light (NF-L, ~68 kDa), medium (NF-M, ~160 kDa) and heavy (NF-H, ~200 kDa), which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 160, also known as NF-M or Neurofilament triplet M protein.

免疫原

neurofilaments purified from pig spinal cord

應用

Anti-Neurofilament 160 antibody has been used in immunoblotting, Immunohistochemistry, Immunofluorescence

生化／生理作用

NFs can accumulate in large numbers within cell bodies and proximal axons of affected neurons in a variety of pathologies, including Charcot-Marie-Tooth disease (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, NFs accumulation were detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. Elevated concentrations of Neurofilament 160 have been shown in cerebrospinal fluid (CSF) and serum samples from patients with brain injury.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable