SAB4200543

Anti-Calbindin-D-28K antibody, Mouse monoclonal

clone CB-955, purified from hybridoma cell culture

• 别名: Monoclonal Anti-(28kD), Monoclonal Anti-D-28K, Monoclonal Anti-D-dependent calcium-binding protein, Monoclonal Anti-RTVL-H protein, Monoclonal Anti-avian-type, Monoclonal Anti-calbindin 1, Monoclonal Anti-vitamin
• UNSPSC代码: 12352203
• NACRES: NA.44

属性

• 生物来源: mouse
• 质量水平: 200
• 偶联物: unconjugated
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: CB-955, monoclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~28 kDa
• 种属反应性: mouse, human, rat
• 增强验证: independent; Learn more about Antibody Enhanced Validation
• 浓度: ~1.0 mg/mL
• 技术: immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum.; western blot: 2-4 μg/mL using MDBK extract.
• 同位素/亚型: IgG1
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... CALB1(793)

说明

一般描述

Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K.

Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca²⁺ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca^2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells.

免疫原

purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

应用

• Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in:
• western blotting
• enzyme-linked immunosorbent assay (ELISA){31
• immunohistochemistry
• immunohistochemical procedure
• indirect immunofluorescence

生化/生理作用

Calbindin D-28k might play a vital role as an intracellular Ca²⁺ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson′s disease (PD).

Calbindin-D (28K) functions as a mobile or partly immobilized Ca²⁺ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca²⁺- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca²⁺-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable