immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect immunofluorescence: suitable western blot: 1:1,000-1:2,000 using whole extracts of human HEK-293T or MCF-7 cells
Monoclonal Anti-Pontin (mouse IgG1 isotype) is derived from the hybridoma 5G3-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human pontin fusion protein. Pontin and reptin are two related members of the AAA+ (ATPases associated with diverse cellular activities) family of ATPases. Pontin and reptin share some homology to the bacterial RuvB gene.
免疫原
human Pontin fusion protein
应用
Monoclonal Anti-Pontin antibody produced in mouse has been used in:
western blotting
immunoprecipitation
ChIP-sequencing
immunohistochemistry
immunofluorescence
RNA immunoprecipitation
生化/生理作用
Pontin and Reptin are associated with several chromatin-remodeling complexes and are involved in multiple biological processes including chromatin remodeling, DNA damage repair, transcriptional regulation, telomerase activity, apoptosis and cancer metastasis. Both proteins are also involved in cellular transformation by beta-catenin and c-myc through their chromatin remodeling function.
外形
Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Primate-specific NBL2 macrosatellite is hypomethylated in several types of tumors, yet the consequences of this DNA hypomethylation remain unknown. We show that NBL2 conserved repeats are close to the centromeres of most acrocentric chromosomes. NBL2 associates with the perinucleolar region
Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression
Tarangelo A, et al.
Nature Communications, 6(6), 10028-10028 (2015)
A novel long non-coding RNA from NBL2 pericentromeric macrosatellite forms a perinucleolar aggregate structure in colon cancer
