跳转至内容
Merck

S9915

Sigma-Aldrich

干细胞因子 来源于小鼠

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

别名:

SCF, c-Kit ligand

登录查看公司和协议定价


About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.77

product name

干细胞因子 来源于小鼠, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

生物源

mouse

品質等級

重組細胞

expressed in E. coli

化驗

≥95% (SDS-PAGE and HPLC)

形狀

powder

效力

≤10 ng/mL ED50/EC50

品質

endotoxin tested

分子量

18.3 kDa

包裝

pkg of 10 μg
pkg of 50 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

雜質

<1 EU/μg

顏色

white

溶解度

water: soluble

UniProt登錄號

儲存溫度

−20°C

基因資訊

一般說明

Stem cell factor (SCF), also known as the c-kit ligand, is produced by the gastrointestinal smooth muscle cells, enteric neurons, endothelial cell, lung fibroblast and bone marrow adipocytes. The SCF gene is mapped to murine chromosome 10.
Recombinant mouse stem cell factor (SCF) is a 18.3 kDa protein containing 164 amino acid residues.

應用

SCF, also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Stem Cell Factor from mouse has been used in the evaluation of in vitro 5-lipoxygenase inhibition by measuring the formation of LTC4.

生化/生理作用

Stem cell factor is responsible for cellular development, proliferation, migration and survival, specially observed with cardiac stem cells. This phenomenon is mediated by stem cell factor (SCF) through chemotaxis. SCF is involved in repair mechanism of myocardial infarctions. SCF signalling is responsible for the function and survival rate of interstitial cells of Cajal. Decreased level of SCF leads to gastrointestinal motility disorders. SCF might be associated with systemic vascular remodeling. SCF signalling is also believed to contribute to the pathogenesis of pulmonary hypertension.
Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

外觀

Lyophilized from a 0.2 μm filtered buffered solution.

分析報告

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

SCF/c-kit transactivates CXCR4-serine 339 phosphorylation through G protein-coupled receptor kinase 6 and regulates cardiac stem cell migration
Zuo K, et al.
Scientific Reports, 6: 26812, 1-12 (2016)
PP2A regulates SCF-induced cardiac stem cell migration through interaction with p38 MAPK
Wang Y, et al.
Life Sciences, 191, 59-67 (2017)
Synthesis and evaluation of benzoxazole derivatives as 5-lipoxygenase inhibitors
Song H, et al.
Bioorganic & Medicinal Chemistry, 18, 7580-7585 (2010)
Antagonism of Stem Cell Factor/c-kit Signaling Attenuates Neonatal Chronic Hypoxia-Induced Pulmonary Vascular Remodeling
Young KC, et al.
Pediatric Research, 79(4), 637-646 (2016)
Persistent distention of colon damages interstitial cells of Cajal through Ca2+-ERK-AP-1-miR-34c-SCF deregulation
Yang S, et al.
Journal of Cellular and Molecular Medicine, 21(9), 1881-1892 (2017)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门