推荐产品
物種活性
human
包裝
kit of 96 wells (12 strips x 8 wells)
技術
ELISA: suitable
capture ELISA: suitable
輸入
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1.37-1000 pg/mL
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... FASLG(356)
一般說明
The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.
免疫原
Recombinant Human Fas Ligand
應用
请参考Protocol了解详情。
生化／生理作用
Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).
其他說明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
訊號詞
Warning
危險聲明
防範說明
危險分類
Met. Corr. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
Yin Li et al.
International journal of clinical and experimental pathology, 8(9), 11915-11920 (2015-12-01)
To investigate the relation of Fas and Fas ligand (FasL) protein expression with carcinogenesis and metastasis of cardiac carcinoma. Immunohistochemistry was used to detect Fas and FasL protein expression in 64 cardiac carcinoma tissue samples and 20 normal gastric tissue
O Micheau et al.
The Journal of biological chemistry, 274(12), 7987-7992 (1999-03-13)
Trimerization of the Fas receptor (CD95, APO-1), a membrane bound protein, triggers cell death by apoptosis. The main death pathway activated by Fas receptor involves the adaptor protein FADD (for Fas-associated death domain) that connects Fas receptor to the caspase
W C Powell et al.
Current biology : CB, 9(24), 1441-1447 (1999-12-23)
The Fas ligand/Fas receptor (FasL/Fas) system is an important mediator of apoptosis in the immune system where the juxtaposition of cells expressing the cell-surface ligand induces the apoptotic pathway in Fas-expressing lymphocytes. The FasL/Fas system has also been shown to
M R Alderson et al.
The Journal of experimental medicine, 181(1), 71-77 (1995-01-01)
A significant proportion of previously activated human T cells undergo apoptosis when triggered through the CD3/T cell receptor complex, a process termed activation-induced cell death (AICD). Ligation of Fas on activated T cells by either Fas antibodies or recombinant human
Çağman Tan et al.
The Turkish journal of pediatrics, 57(2), 141-145 (2015-12-23)
Defects in genes that have role in apoptotic pathways result in development of Autoimmune Lymphoproliferative Syndrome (ALPS) and ALPS related disorders. Germline and somatic FAS mutations, FASL and CASP10 mutations constitute other genetic defects in ALPS. Patients who fulfill ALPS
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