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R8755

Sigma-Aldrich

RPMI-1640 培养基

With ʟ-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

别名:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL號碼:
MFCD00217820
分類程式碼代碼:
12352207
NACRES:
NA.75

product name

RPMI-1640 培养基, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

形狀

powder

技術

cell culture | mammalian: suitable

成分

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

應用

RPMI-1640培养基被用于以下应用：
  • 用于细胞培养基：补充其他组分的RPMI-1640培养基可用于培养鼠胸腺瘤EL-4细胞
  • 不含抗生素的RPMI 1640培养基可用于脾细胞的分枝杆菌感染
  • 用于细胞分离和培养
  • 作为培养基组分之一，用于淋巴细胞分离和培养、ELISpot酶联免疫斑点测定、离体增殖试验
  • 作为培养基，用于gp39基因培养和CD40免疫球蛋白结合试验
  • 抗真菌剂制备

數量

按照每升培养基10.4克粉末配制。

重構

补充2.0 g/L碳酸氢钠。

其他說明

酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于干细胞培养或低密度的细胞培养。也推荐用于体外诊断。

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補充物

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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RPMI-1640 培养基

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ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

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ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

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ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

实验方案

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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