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Merck
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PP0510

Sigma-Aldrich

GlycoProfile™ IV 化学法去糖基化试剂盒

TFMS Deglycosylation System

别名:

Deglycosylation kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

200

儲存溫度

2-8°C

相关类别

一般說明

GlycoProfile™ IV 试剂盒使用了去糖基化系统中的三氟甲磺酸（TFMS），可完全去除N- 和O-连接聚糖，同时保护蛋白质或多肽结构。TFMS作用下的糖蛋白水解使得蛋白质分解量达到最小，同时消除了聚糖的释放。反应条件经过优化，无需清除剂即可在30分钟内使简单或低分子量糖蛋白脱糖基。对于高分子量或复杂的非哺乳动物糖蛋白，以无水茴香醚为清除剂可以得到高蛋白质产率。

應用

GlycoProfile™ IV 化学去糖基化试剂盒已被用于人αL-艾杜糖苷酸酶和来自棉铃虫肠道的围食膜基质（peritrophic matrix ）的去糖基。它已被用于评估植物重组人白细胞介素13（rhIL-13）的糖基化。

特點和優勢

  • 消除蛋白质中的N- 和O-连接聚糖
  • 便于我们通过质谱（MS）进行蛋白核心的序列分析或DNA至蛋白序列比较
  • 消除影响分子半径的聚糖
  • 便于我们通过电泳进行分子量测定
  • 在30分钟内完成高通量去糖基化
  • 反应快速因为无需进行多种酶反应
  • 适合各种糖蛋白
  • 蛋白核心（ protein core）降解最少以便获取可靠的MS结果

數量

该试剂盒含有可以用于10次样品大小在1-2 mg的典型糖蛋白反应所需的试剂。其中还包括了RNase B糖蛋白标准品。

法律資訊

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Trifluoromethanesulfonic Acid (TFMS) 5 x 1
  • RNase B Glycoprotein Standard 3 x 1
  • Pyridine solution, 60% 10 mL
  • Bromophenol blue solution, 0.2% .5 mL
  • Anisole, anhydrous 5 x 1
  • Reaction vials 10 ea

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 1 - STOT SE 3

標靶器官

Eyes,Central nervous system, Respiratory system

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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A novel platform for biologically active recombinant human interleukin-13 production
David J W, et al.
Plant Biotechnology (2008)
Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera
Peter M C, et al.
Insect Biochemistry and Molecular Biology, 950-958 (2008)
Production and characterization of soluble human lysosomal enzyme alpha-iduronidase with high activity from culture media of transgenic tobacco BY-2 cells
Fu L H, et al.
Plant Science, 177(6), 668-675 (2009)

商品

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

实验方案

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

Mass Spectrometry of Glycans

Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.

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