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形狀
ammonium sulfate suspension
比活性
5.0-20.0 units/mg protein (biuret)
儲存溫度
2-8°C
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一般說明
丙酮酸脱羧酶(PDC)通常出现在萌发阶段的植物种子中,特别是当整个植物胚都包裹氧透过性种皮时。
應用
丙酮酸脱羧酶(PDC)可用于研究参与硫胺素焦磷酸(TPP)结合的残基。 也可用于研究植物发酵途径调控。
丙酮酸脱羧酶(来自面包酵母/酿酒酵母)已用于评估有益代谢酶调控系统鉴定(SIMMER,systematic identification of meaningful metabolic enzyme regulation)方法发现未知酵母调控作用的能力。
生化/生理作用
丙酮酸脱羧酶(PDC)是可在细胞质中催化丙酮酸脱羧成为乙醛和二氧化碳的同四聚体酶。 丙酮酸脱羧酶(PDC)依赖辅因子硫胺素焦磷酸(TPP)和镁。 PDC具有1个β-α-β结构,形成平行β-折叠片。
丙酮酸脱羧酶(PDC)活跃参与多种细菌、酵母和植物种子的厌氧代谢。
單位定義
在pH 6.0、25℃条件下,一个单位每分钟可将1.0 μmole丙酮酸转化为乙醛。
外觀
溶于3.2 M (NH4)2SO4(pH 6.5)的悬液,添加5%甘油、5 mM磷酸钾、1 mM乙酸镁、0.5 mM EDTA和25 μM辅羧酶帮助稳定。
準備報告
分离纯化处理时不曾使用重金属。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
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Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described. xpkA encodes a 788-amino-acid protein with
Annals of botany, 91 Spec No, 291-300 (2003-01-02)
Submergence tolerance of 13 doubled haploid lines of rice and their parents (submergence tolerant FR13A and submergence intolerant CT6241) was assessed using 2-week-old seedlings. Plants were scored for leaf senescence and percentage of seedlings that survived up to 15 d
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Science, 354(6311), aaf2786-aaf2786 (2016)
Substrate activation behaviour of pyruvate decarboxylase from Pisum sativum cv. Miko
Febs Letters, 400(1), 42-44 (1997)
Biochemistry, 32(24), 6165-6170 (1993-06-22)
The crystal structure of brewers' yeast pyruvate decarboxylase, a thiamin diphosphate dependent alpha-keto acid decarboxylase, has been determined to 2.4-A resolution. The homotetrameric assembly contains two dimers, exhibiting strong intermonomer interactions within each dimer but more limited ones between dimers.
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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