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Merck
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文件

P9187

Sigma-Aldrich

OPD

SIGMAFAST™, peroxidase substrate, chromogenic, tablet

别名:

OPD过氧化物酶底物

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ OPD, tablet

形狀

tablet

溶解度

water: 0.5 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

相关类别

應用

SIGMAFAST™ OPD (o-苯二胺二盐酸盐)片剂用作酶免疫测定(EIA)检测氧化物酶活性的可溶底物。OPD作为EIA底物是因为它有高灵敏度。 使用OPD的EIA可以在计时分析中读数或用稀释的酸性溶液终止反应，过后在读数。
SIGMAFAST OPD已用于唾液病毒样颗粒(VLP)结合测定。它还已用于开发检测大鼠血清样品中的利妥昔单抗的酶联免疫吸附测定(ELISA)板。

特點和優勢

SIGMAFAST™ OPD片剂不需要另外的缓冲液或步骤来制备活性底物溶液。

注意

该底物吸湿且光敏感

重構

一片OPD片剂和一片尿素过氧化氢/缓冲液片剂，溶解在20ml水中，提供20ml即用底物。 如果按照该方法溶解片剂，得到的终浓度是0.4 mg/ml OPD, 0.4 mg/ml尿素过氧化氢和0.05 M磷酸柠檬酸盐，pH5.0。片剂是独立铝膜包装。
将每组片剂溶解在20mL去离子水中，就能生成即用型含有OPD和尿素过氧化氢缓冲溶液。

法律資訊

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

分析证书(COA)

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Elisa Lobato et al.
Ecology and evolution, 7(11), 3645-3656 (2017-06-16)
Oceanic islands share several environmental characteristics that have been shown to drive convergent evolutionary changes in island organisms. One change that is often assumed but has seldom been examined is the evolution of weaker immune systems in island species. The
Courtney Woolsey et al.
Nature immunology, 22(1), 86-98 (2020-11-26)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen vaccines and treatments. We show that African green monkeys (AGMs)
Eliana Spilioti et al.
Environmental geochemistry and health, 39(4), 821-833 (2016-07-23)
Spa resorts are known for thousands of years for their healing properties and have been empirically used for the treatment of many inflammatory conditions. Mud is one of the most often used natural materials for preventive, healing and cosmetic reasons
Jeffrey D Whitman et al.
Nature biotechnology, 38(10), 1174-1183 (2020-08-29)
Appropriate use and interpretation of serological tests for assessments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity require accurate data on assay performance. We conducted a head-to-head evaluation of ten point-of-care-style lateral flow assays (LFAs)
Almahamoudou Mahamar et al.
Malaria journal, 16(1), 289-289 (2017-07-20)
Seasonal malaria chemoprevention (SMC) is a new strategy to reduce malaria burden in young children in Sahelian countries. It consists of the administration of full treatment courses of sulfadoxine-pyrimethamine plus amodiaquine to children at monthly intervals during the malaria season.
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