Using P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde was attempted, but the results were not satisfactory with regular formaldehyde. A specific protocol for the reconstitution of Phalloidin P5282-.1MG in the case of tissues fixed in paraformaldehyde and included in O.C.T medium is not available. However, it is suggested to find relevant information in the literature, such as in this article, https://www.oncotarget.com/article/25259/ DOI:[10.18632/oncotarget.25259].
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一般描述
应用
警示用语：
Danger
危险分类
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Non-muscle myosin heavy chain as a possible target for protein encoded by metastasis-related mts-1 gene.
Kriajevska MV
The Journal of Biological Chemistry, 19679-82, 269(31)-269(31) (1994)
Fluorescent phallotoxin, a tool for the visualization of cellular actin.
Wulf E
Proceedings of the National Academy of Sciences of the USA, 76(9), 4498-4502 (1979)
Ting Xu et al.
Journal of leukocyte biology, 84(4), 1192-1201 (2008-07-26)
Recruitment of leukocytes onto inflamed tissues is an important physiological event, in which L-selectin plays an essential role in initial leukocyte capture and at the same time, triggers cell signaling. Lck is a member of the Src family of protein
The H9C2 cell line and primary neonatal cardiomyocyte cells show similar hypertrophic responses in vitro.
Watkins SJ
In Vitro Cellular & Developmental Biology. Animal, 47(2), 125-131 (2011)
The culture of fibroblasts from diaphragm of giant panda.
Han ZM
In Vitro Cellular & Developmental Biology. Animal, 37(10), 644-645 (2001)
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在实验室计时器蜂鸣不止、报告和授权截止期限迫在眉睫时，很容易放松对实验室试剂质量的授权。
In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
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Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
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Is it possible to use P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde?
Is it possible to use P5282 with cells fixed in paraformaldehyde instead of methanol-free formaldehyde?
1 answer-
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How do I stain cells with Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
A typical application for staining cells can be found in the product information sheet (under Documents, above).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How do I dissolve Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
This product is soluble in methanol, ethanol, butanol and pyridine. It is soluble in cold water at a concentration of 5 mg/mL; it is much more soluble in hot water. See: The chemicals encyclopedia published by the Royal Society of Chemistry, 12th ed., entry# 7336 (1996). Solutions should be prepared fresh, and protected from light whenever possible.
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How do I prepare stock solutions of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1 to 5 mg/mL. Make final dilutions in aqueous physiological buffers for a staining range from 0.1 μM to 100 μM with corresponding incubation times of 15 minutes to 72 hours.
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What are the excitation and emission wavelengths used for fluorescent detection of Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
Excitation wavelengths of 540-545 nm and emission wavelengths of 570-573 nm can be used. See: Faulstich, H., J. Muscle Res. Cell Motility, 9, 370 (1988), and Waggoner, A. et al., Methods in Cell Biology, 30, 449 (1989).
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How do I store Product P5282, Phalloidin, Fluorescein Isothiocyanate Labeled?
1 answer-
The product should be stored at a powder in the freezer at -20°C.
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