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Merck
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文件

P4716

Sigma-Aldrich

果胶酶 来源于黑曲霉

greener alternative

BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)

别名:

多聚半乳糖醛酸酶 溶液 来源于黑曲霉, 聚-（1,4-& # 945;-D·-半乳糖醛酸）聚糖水解酶

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About This Item

CAS号:
9032-75-1
EC 号:
3.2.1.15 (BRENDA, IUBMB)
EC號碼:
232-885-6
MDL號碼:
MFCD00131809
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Aspergillus niger

產品線

BioReagent

形狀

aqueous glycerol solution

比活性

≥5 units/mg protein (Lowry)

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

cell culture | plant: suitable

環保替代類別

, Enabling

儲存溫度

2-8°C

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一般說明

我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对纤维素乙醇研究进行了优化，可提高能效并防止浪费。有关详细信息，请参阅《biofiles》中的文章和《替代能源研究用酶》

應用

黑曲霉来源果胶酶可用于：
  • 原生质体释放
  • 原生质体酶混合物，进行酶解
  • 糖的部分糖化
  • 研究其在植物病原菌入侵植物组织、农产品变质、各种食品加工和植物生物技术应用中的作用

用于植物原生质体制备，在细胞器分离前消化细胞壁。

生化／生理作用

从黑曲霉中选定菌株生成的果胶酶制剂：主要含有果胶转移酶、多聚半乳糖醛酸酶和果胶酯酶以及少量的半纤维素酶和纤维素酶。果胶酶水解果胶，果胶是细胞壁的一个组成部分。它们可能攻击甲基酯化果胶或去酯化果胶。它是果胶酶活性的来源，还含有纤维素酶和半纤维素酶活性。果胶酶催化果胶和其他半乳糖醛酸中的a-（1-4）-Dgalactosiduronic键的随机水解。

單位定義

在25°C条件下，当pH为4.0时，一个单位每分钟将释放从多聚半乳糖醛酸中得到的1.0 μ摩尔的半乳糖醛酸。

外觀

40%甘油溶液

其他說明

有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

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Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand
Chokchaichamnankit P, et al.
Thai Forest Bulletin , 38-44 (2007)
Tony Heitkam et al.
The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)
If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on
Tolerance mechanisms in mercury-exposed Chromolaena odorata (lf) RM King et H. Robinson, a potential phytoremediator
Alcantara HJP, et al.
Journal of Degraded and Mining Lands Management, 1(1), 09-20 (2013)
Sima Taheri et al.
International journal of molecular sciences, 23(13) (2022-07-10)
Boesenbergia rotunda (Zingiberaceae), is a high-value culinary and ethno-medicinal plant of Southeast Asia. The rhizomes of this herb have a high flavanone and chalcone content. Here we report the genome analysis of B. rotunda together with a complete genome sequence
Nicola Schmidt et al.
Annals of botany, 128(3), 281-299 (2021-03-18)
Endogenous pararetroviruses (EPRVs) are widespread components of plant genomes that originated from episomal DNA viruses of the Caulimoviridae family. Due to fragmentation and rearrangements, most EPRVs have lost their ability to replicate through reverse transcription and to initiate viral infection.
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实验方案

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

Enzymatic Assay of Pectinase

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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