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Merck

P3563

Sigma-Aldrich

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

别名:

1X PBS + 0.05% TWEEN® 20, PBS-T

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About This Item

分類程式碼代碼:
41105319
eCl@ss:
32129211
NACRES:
NA.25

product name

磷酸盐缓冲剂,含0.05% TWEEN® 20,pH 7.4,

形狀

powder

溶解度

water: soluble, clear, colorless (1 pouch dissolved in 1L)

應用

cell analysis
diagnostic assay manufacturing

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一般說明

磷酸盐缓冲液-Tween®20(PBST)主要用作免疫分析中的洗涤和封闭缓冲液。磷酸缓冲液用于维持稳定的pH 7.4,适合许多生物反应和试验。PBS-T中的盐或生理盐溶液用于提供维持最佳蛋白溶解度和稳定性需要的离子强度。0.05% TWEEN®-20是一种非离子去污剂,用于通过封闭蛋白的非特异性结合位点降低背景信号。PBST缓冲液通过清除不必要物质提高洗涤效率,并在保持靶蛋白完整性的前提下控制非特异性抗体结合位点。

應用

磷酸盐缓冲液用于:

  • 蛋白质免疫印迹分析
  • 酶联免疫吸附测定(ELISA)
  • 端粒FISH (Tel-FISH)和端粒/免疫染色-FISH (Teli-Fish)
  • 细胞分离

包裝

锡箔袋包装

重構

一个袋中的内容物溶解于1L蒸馏水或超纯水水中,将得到0.01 M磷酸盐缓冲液、0.138M NaCl、0.0027M KCl、0.05% Tween 20,pH7.4.

其他說明

如需了解生化试剂系列的更多信息,请填写此表

法律資訊

TWEEN is a registered trademark of Croda International PLC

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Mass drug administration (MDA) of ivermectin has become the main intervention to control onchocerciasis or "river blindness". In Togo, after many years of MDA, Onchocerca volvulus infection has declined dramatically, and elimination appears achievable, but in certain river basins the
Shorter telomeres in luminal B, HER-2 and triple-negative breast cancer subtypes
Heaphy C M, et al.
Modern Pathology, 24(2), 194-194 (2011)
The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
Subhawong A P, et al.
Modern Pathology, 22(11), 1423-1423 (2009)
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)

实验方案

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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