推荐产品
生物源
rat
品質等級
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
NZ-1.2, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~36 kDa
物種活性
human
濃度
~1.5 mg/mL
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 4-8 μg/mL using U87 total cell extract
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... PDPN(10630)
一般說明
Monoclonal Anti-Podoplanin (rat IgG2a isotype) is derived from the hybridoma NZ-1.2 produced by the fusion of mouse myeloma cells and splenocytes from CD rat immunized with platelet-aggregation-stimulating (PLAG) domain of podoplanin. Podoplanin (Aggrus) is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. The extracellular domain possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has been described to be present in normal, inflammatory and neoplastic lymphatic capillary endothelium.
免疫原
platelet-aggregation-stimulating (PLAG) domain of human podoplanin.
應用
Anti-Podoplanin antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- flow cytometry
- immunocytochemistry
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
生化／生理作用
Expression of podoplanin is regulated by the lymphatic-specific homeobox gene Prox-1 (prospero-1). It is involved in lymphatic vessel formation and might be associated with tissue development and repair. Podoplanin is detected in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. It is implicated in the onset of tumor invasion, metastasis or malignant progression. Podoplanin plays an important role in the maintenance of podocyte foot processes and glomerular permeability.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Peter Birner et al.
Cancer, 120(16), 2440-2447 (2014-04-29)
Recently, isocitrate dehydrogenase 1 (IDH1) was identified as a major participant in glioma pathogenesis. At present, the enzymatic activity of the protein has been the main topic in investigating its physiological function, but its signaling pathway allocation was unsuccessful. Interestingly
Hao Xue et al.
Autophagy, 12(7), 1129-1152 (2016-05-11)
Hypoxia induces protective autophagy in glioblastoma cells and new therapeutic avenues that target this process may improve the outcome for glioblastoma patients. Recent studies have suggested that the autophagic process is upregulated in glioblastomas in response to extensive hypoxia. Hypoxia
Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration
Ochoa-Alvarez JA, et al.
Testing, 7(7), e41845-e41845 (2012)
S Breiteneder-Geleff et al.
The American journal of pathology, 151(4), 1141-1152 (1997-11-05)
Puromycin aminonucleoside nephrosis (PAN), a rat model of human minimal change nephropathy, is characterized by extensive flattening of glomerular epithelial cell (podocyte) foot processes and by severe proteinuria. For comparison of expression of glomerular membrane proteins of normal and PAN
The potential role of podoplanin in tumour invasion
Wicki A and Christofori G
British Journal of Cancer, 96(1), 1-1 (2007)
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