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等級
for molecular biology
形狀
liquid
技術
DNA amplification: suitable
DNA extraction: suitable
儲存溫度
−20°C
一般說明
这种中和缓冲液B要与 Sigma′REDExtract-N-Amp™ 组织 PCR.试剂盒一起使用。该缓冲液用于中和DNA提取液，并与REDExtract-N-Amp PCR 反应混合液及PCR引物一起用于扩增靶标DNA。 REDExtract-N-Amp试剂盒包括足够体积的中和缓冲液B，每次提取至少可中和一次。 不过，如果需要更多的扩增反应，则可能需要补充额外的中和缓冲液。
應用
中和缓冲液B 已被用于改良的 Sigma Extract-N-Amp ™ 提取程序。它也用于小鼠的基因分型，用于聚合酶链反应 (PCR) 之前使用Sigma′的REDExtract-N-Amp™ PCR 反应混合物来中和DNA提取液。
法律資訊
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Warning
危險分類
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Işın Çakır et al.
Nature metabolism, 4(1), 44-59 (2022-01-19)
The adipose tissue-derived hormone leptin can drive decreases in food intake while increasing energy expenditure. In diet-induced obesity, circulating leptin levels rise proportionally to adiposity. Despite this hyperleptinemia, rodents and humans with obesity maintain increased adiposity and are resistant to
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish
Varshney GK, et al.
Nature Protocols, 11(12), 2357-2357 (2016)
Jun He et al.
Pharmacology, biochemistry, and behavior, 86(2), 327-333 (2006-12-16)
Adolescence is an important stage of brain development. Recent studies have indicated that neurogenesis in the brain occurs throughout life prompting comparisons of adolescent and adult neurogenesis. Since insulin-like growth factor 1 (IGF-1) has been implicated in promoting neurogenesis we
Yan Zhang et al.
American journal of physiology. Renal physiology, 319(6), F1135-F1148 (2020-11-10)
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the presence of numerous fluid-filled cysts, extensive fibrosis, and the progressive decline in kidney function. Transforming growth factor-β1 (TGF-β1), an important mediator for renal fibrosis and chronic kidney disease, is overexpressed
实验方案
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.
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